It is overexpressed in the neutrophil and T lymphocytes. Actin is frequently found in the cytoplasmic mRNP granules containing untranslated mRNAs and is expressed in the whole blood and adrenal gland. Monoclonal mouse anti-actin antibody was used. Background Beta-Actin is a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism. Rabbit polyclonal antibody to beta actin, which is also known as PS1TP5BP1 and BRWS1. Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates. Polyclonal antibodies are purified by peptide affinity chromatography Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected beta-Actin in A431 cell lysate. Beta-actin is highly conserved among species.
#BETA ACTIN KDA WESTERN BLOT FULL#
Proteintech’s monoclonal beta-actin antibody (Figure 1) recognizes all fragments, while 66009-1-Ig antibody (Figure 2) can only detect the full length of beta-actin. Recombinant human beta actin protein (orb84841) was used as immunogen. Suitable for use as control antibody for Actin siRNA (h): sc-29191 and Actin. Evaluated by Western Blotting in A431 cell lysate. Though beta-actin has a molecular weight of 42 kDa, degradation by apoptosis will produce 38-41 kDa fragments on a Western blot.
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